As can be seen in these images, the edges of the cell are clearly visible and the acrosome can be easily distinguished from the nucleus. Type of animal cell. In some countries this may not be legally possible; if so: Thus, in the resulting image, the birefringent regions are brighter than other regions and the background is completely dark. High-resolution images of bovine sperm have been obtained by DH microscopy from the reconstruction of a single acquired hologram, highlighting in some cases morphological alterations. Overlaying these two images produces an interference image as in DIC, yet with very different results.
In vitro evaluation of sperm quality:
File:Complete diagram of a human spermatozoa en.svg
For this purpose, an experimental set-up combining digital holography DH microscopy and Raman spectroscopy RS has been developed. This technique offers a huge potential for solving biomedical problems, such as single-cell characterization [ 25 ], biomarkers detection [ 26 ] and sex-identification for instance [ 3 ], as demonstrated by the recent increase in this field publications [ 25262728 ]. Interferometric phase microscopy IPMon the other hand, is able to provide a quantitative light delay map for all spatial points of the imaged sperm. The following other wikis use this file: Views View Edit History.